Dr. Robert Hertel
Address
Georg-August University of Göttingen   Institute of Microbiology and Genetics   Department of Genomic and Applied Microbiology   Grisebachstr. 8, 37077 Göttingen, Germany
Office
Room:
Tel: ++49 (0)551 39
E-Mail: 0(at)b-tu.de

Curriculum Vitae

Major Research Interests

• Phage biology: What is out there: investigating phage diversity based on Bacillus subtilis
  Bacteriophages are viruses of bacteria. In this project we like to explore the abundance, as well as the morphological and genetically diversity of phages from differed habitats infecting the soil bacterium Bacillus subtilis. The findings should lead to a better understanding of phage biology and will serve as a source of information for phage based applications.

Research Project(s)

• When two phages quarrel does the bacterium rejoices?

• A twofold retrosynthetic implementation of a novel biochemical pathway, a synthetic route to biotin

Publications

Riedel R, Commichau FM, Benndorf D, Hertel R, Holzer K, Hoelzle LE, Mardoukhi MSY, Noack LE, Martienssen M (2024) Biodegradation of selected aminophosphonates by the bacterial isolate Ochrobactrum sp. BTU1. Microbiol Res 280:127600 (doi:10.1016/j.micres.2024.127600).

Chibani CM, Hertel R, Neumann-Schaal M, Goehlich H, Wagner K, Bunk B, Spröer C, Overmann J, Hoppert M, Marten M, Roth O, Liesegang H, Wendling CC (2023) Vibrio syngnathi sp.nov., a fish pathogen, isolated from the Kiel fjord. Int J Syst Evol Microbiol 73(6):005951 (doi:10.1099/ijsem.0.005951).

Commichau FM, Hertel R, Stülke J (2023) Projekt im Wandel: von iGEM zu neuen Ufern. BIOspektrum 29:698

Friedrich I, Hertel R (2023) Isolation of a host-confined phage metagenome allows the detection of phages both capable and incapable of plaque formation. Methods Mol Biol 2555:195-203 (doi:10.1007/978-1-0716-2795-2_14).

Friedrich I, Kuritsyn A, Hertel R, Daniel R (2023) Luteibacter flocculans sp. nov., isolated from a eutrophic pond and isolation and characterization of Luteibacter phage vB_LflM‐Pluto. Microorganisms 11:307 (doi:10.3390/ microorganisms11020307).

Friedrich I, Neubauer H, Kuritsyn A, Bodenberger B, Tskhay F, Hartmann S, Poehlein A, Bömeke M, Hoppert M, Schneider D, Hertel R, Daniel R (2023) Brevundimonas and Serratia as host systems for assessing associated environmental viromes and phage diversity by complementary approaches. Front Microbiol 14:1095850 (doi:10.3389/fmicb.2023.1095850).

Kohm K, Lutz VT, Friedrich I, Hertel R (2023) CRISPR-Cas9 shaped viral metagenomes associated with Bacillus subtilis. Methods Mol Biol 2555:205-212 (doi:10.1007/978-1-0716-2795-2_15).

Kohm K, Jalomo-Khayrova E, Krüger A, Basu S, Steinchen W, Bange G, Frunzke J, Hertel R, Commichau FM, Czech L (2023) Structural and functional characterization of MrpR, the master repressor of the Bacillus subtilis prophage SPβ. Nucleic Acids Res 51(17):9452-9474 (doi:10.1093/nar/gkad675).

Schwedt I, Collignon M, Mittelstädt C, Giudici F, Rapp J, Meißner J, Link H, Hertel R, Commichau FM (2023) Genomic adaptation of Burkholderia anthina to glyphosate uncovers a novel herbicide resistance mechanism. Environ Microbiol Rep 15(6):727-739 (doi:10.1111/1758-2229.13184).

Schwedt I, Schöne K, Eckert M, Pizzinato M, Winkler L, Knotkova B, Richts B, Hau JL, Steuber J, Mireles R, Noda-Garcia L, Fritz G, Mittelstädt C, Hertel R, Commichau FM (2023) The low mutational flexibility of the EPSP synthase in Bacillus subtilis is due to a higher demand for shikimate pathway intermediates. Environ Microbiol 25(12):3604-3622 (doi:10.1111/1462-2920.16518).

Hertel R, Schöne K, Mittelstädt C, Meißner J, Zschoche N, Collignon M, Kohler C, Friedrich I, Schneider D, Hoppert M, Kuhn R, Schwedt I, Scholz P, Poehlein A, Martienssen M, Ischebeck T, Daniel R, Commichau FM (2022) Characterization of glyphosate-resistant Burkholderia anthina and Burkholderia cenocepacia isolates from a commercial Roundup® solution. Environ Microbiol Rep 14(1):70-84 (doi:10.1111/1758-2229.13022).

Hertel R, Gibhardt J, Martienssen M, Kuhn R, Commichau FM (2022) Molecular mechanisms underlying glyphosate resistance in bacteria. Environ Microbiol 23(6):2891-2905 (doi:10.1111/1462-2920.15534).

Kohm K, Floccari VA, Lutz VT, Nordmann B, Mittelstädt C, Poehlein A, Dragoš A, Commichau FM, Hertel R (2022) The Bacillus phage SPβ and its relatives: a temperate phage model system reveals new strains, species, prophage integration loci, conserved proteins and lysogeny management components. Environ Microbiol 24(4):2098-2118 (doi:10.1111/1462-2920.15964).

Schmitt M, Jarosch KA, Hertel R, Spielvogel S, Dippold MA, Loeppmann S (2022) Manufacturing triple-isotopically labeled microbial necromass to track C, N and P cycles in terrestrial ecosystems. Appl Soil Ecol 171:104322 (doi:10.1016/j.apsoil.2021.104322).

Aloo BN, Mbega ER, Makumba BA, Hertel R, Daniel R (2021) Molecular identification and in vitro plant growth-promoting activities of culturable potato (Solanum tuberosum L.) rhizobacteria in Tanzania. Potato Res 64:67–95 (doi:10.1007/s11540-020-09465-x).

Friedrich I, Hollensteiner J, Scherf J, Weyergraf J, Klassen A, Poehlein A, Hertel R, Daniel R (2021) Complete genome sequence of Stenotrophomonas indicatrix DAIF1. Microbiol Resour Announc 10(6):e01484-20 (doi:10.1128/MRA.01484-20).

Friedrich I, Klassen A, Neubauer H, Schneider D, Hertel R, Daniel R (2021) Living in a puddle of mud: isolation and characterization of two novel Caulobacteraceae strains Brevundimonas pondensis sp. nov. and Brevundimonas goettingensis sp. nov.. Appl Microbiol 1:38-59 (doi:10.3390/applmicrobiol1010005).

Friedrich I, Bodenberger B, Neubauer H, Hertel R, Daniel R (2021) Down in the pond: Isolation and characterization of a new Serratia marcescens strain (LVF3) from the surface water near frog’s lettuce (Groenlandia densa). PLoS One 16(11):e0259673 (doi:10.1371/journal.pone.0259673).

Hertel R (2021) SPβ, ein Bacillus-Phage mit vielen Geheimnissen. BIOspektrum 27:781 (doi:10.1007/s12268-021-1666-5).

Hollensteiner J, Friedrich I, Hollstein L, Lamping J-P,Wolf K, Liesegang H, Poehlein A, Hertel R, Daniel R (2021) Complete genome sequence of Kinneretia sp. strain DAIF2, isolated from a freshwater pond. Microbiol Resour Announc 10(8):e00003-21 (doi:10.1128/MRA.00003-21).

Kohm K, Hertel R (2021) The life cycle of SPβ and related phages. Arch Virol 166:2119-2130 (doi:10.1007/s00705-021-05116-9).

Kohm K, Basu S, Nawaz MM, Hertel R (2021) Chances and limitations when uncovering essential and non‐essential genes of Bacillus subtilis phages with CRISPR‐Cas9. Environ Microbiol Rep 13(6):934-944 (doi:10.1111/1758-2229.13005).

Aloo BN, Mbega ER, Makumba BA, Friedrich I, Hertel R, Daniel R (2020) Whole-genome sequences of three plant growth-promoting rhizobacteria isolated from Solanum tuberosum L. rhizosphere in Tanzania. Microbiol Resour Announc 9(20):e00371-20 (doi:10.1128/MRA.00371-20).

Baena Lozada LP, Hoppert M, Hertel R (2020) Phage vB_BmeM-Goe8 infecting Bacillus megaterium DSM319. Arch Virol 165:515–517 (doi:10.1007/s00705-019-04513-5).

Chibani CM, Hertel R, Hoppert M, Liesegang H, Wendling CC (2020) Closely related Vibrio alginolyticus strains encode an identical repertoire of Caudovirales-like regions and filamentous phages. Viruses 12(12):1359 (doi:10.3390/v12121359).

Egelkamp R, Friedrich I, Hertel R, Daniel R (2020) From sequence to function: a new workflow for nitrilase identification. Appl Microbiol Biotechnol 104:4957–4970 (doi:10.1007/s00253-020-10544-9).

Friedrich I, Hollensteiner J, Schneider D, Poehlein A, Hertel R, Daniel R (2020) First complete genome sequences of Janthinobacterium lividum EIF1 and EIF2 and their comparative genome analysis. Genome Biol Evol 12(10):1782-1788 (doi:10.1093/gbe/evaa148).

Furrer AD, Bömeke M, Hoppert M, Hertel R (2020) Phage vB_BveM-Goe7 represents a new genus in the subfamily Bastillevirinae. Arch Virol 165(4):959-962 (doi:10.1007/s00705-020-04546-1).

Otte K, Kühne NM, Furrer AD, Baena Lozada LP, Lutz VT, Schilling T, Hertel R (2020) A CRISPR‐Cas9 tool to explore the genetics of Bacillus subtilis phages. Lett Appl Microbiol 71(6):588-595 (doi:10.1111/lam.13349).

Richts B, Hertel R, Potot S, Poehlein A, Daniel R, Schyns G, Pragai Z, Commichau FM (2020) Complete genome sequence of the prototrophic Bacillus subtilis subsp. subtilis strain SP1. Microbiol Resour Announc 9(32):e00825-20 (doi:10.1128/MRA.00825-20).

Egelkamp R, Zimmermann T, Schneider D, Hertel R, Daniel R (2019) Impact of nitriles on bacterial communities. Front Env Sci 7:103 (doi:10.3389/fenvs.2019.00103).

Nordmann B, Schilling T, Hoppert M, Hertel R (2019) Complete genome sequence of the virus isolate vB_BthM-Goe5 infecting Bacillus thuringiensis. Arch Virol 164(5):1485-1488 (doi:10.1007/s00705-019-04187-z).

Schilling T, Hoppert M, Daniel R, Hertel R (2018) Complete genome sequence of vB_BveP-Goe6, a virus infecting Bacillus velezensis FZB42. Genome Announc 6(8):e00008-18 (doi:10.1128/genomeA.00008-18).

Schilling T, Hoppert M, Hertel R (2018) Genomic analysis of the recent viral isolate vB_BthP-Goe4 reveals increased diversity of φ29-Like Phages. Viruses 10(11):624 (doi:10.3390/v10110624).

Schilling T, Dietrich S, Hoppert M, Hertel R (2018) A CRISPR-Cas9-based toolkit for fast and precise in vivo genetic engineering of Bacillus subtilis phages. Viruses 10(5):241 (doi:10.3390/v10050241).

Singh N, Herbig E, Hertel R (2018) Enzyme aus der Natur - Reinstwasser als Grundlage für Metagenomik. GIT-Labor 06.03.2018 (abstract)

Egelkamp R, Schneider D, Hertel R, Daniel R (2017) Nitrile-degrading bacteria isolated from compost. Front Env Sci 5:56 (doi:10.3389/fenvs.2017.00056).

Hertel R, Meyerjürgens S, Voigt B, Liesegang H, Volland S (2017) Small RNA mediated repression of subtilisin production in Bacillus licheniformis. Sci Rep 7:5699 (doi:10.1038/s41598-017-05628-y).

Wendling CC, Piecyk A, Refardt D, Chibani C, Hertel R, Liesegang H, Bunk B, Overmann J, Roth O (2017) Tripartite species interaction: eukaryotic hosts suffer more from phage susceptible than from phage resistant bacteria. BMC Evol Biol 17(1):98 (doi:10.1186/s12862-017-0930-2).

Willms IM, Hoppert M, Hertel R (2017) Characterization of Bacillus subtilis viruses vB_BsuM-Goe2 and vB_BsuM-Goe3. Viruses 9(6):146 (doi:10.3390/v9060146).

Wilms IM, Hertel R (2016) Phage vB BsuP-Goe1: the smallest identifed lytic phage of Bacillus subtilis. FEMS Microbiol Lett 363:fnw208 (doi:10.1093/femsle/fnw208).

Hertel R, Volland S, Liesegang H (2015) Conjugative reporter system for the use in Bacillus licheniformis and closely related Bacilli. Lett Appl Microbiol. 60(2):162-167 (doi:doi.org/10.1111/lam.12352).

Hertel R, Rodríguez DP, Hollensteiner J, Dietrich S, Leimbach A Hoppert M, Liesegang H, Volland S (2015) Genome-based identification of active prophage regions by Next Generation Sequencing in Bacillus licheniformis DSM13. PLOS One 10(3):e0120759 (doi:10.1371/journal.pone.0120759).

Wiegand S, Dietrich S, Hertel R, Bongaerts J, Evers S, Volland S, Daniel R, Liesegang H (2013) RNA-Seq of Bacillus licheniformis: active regulatory RNA features expressed within a productive fermentation. BMC Genomics 14:667 (doi:10.1186/1471-2164-14-667).